Ni Seplife® 6FF (NTA) Chromatographic Media

Ni Seplife® 6FF (NTA) Chromatographic Media

Catalog Number:
CM01110675SUN
Mfr. No.:
NiSeplife6FFNTA
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    • Overview
      • Ni Chelating Agarose Chromatography (NTA) is binding iminodiacetic acid on 6FF agarose media and then chelating ion of Ni2+ to be as an affinity chromatographic media. It is widely used for the processing of protein, nucleic acid and polypeptide purification in down stream of biopharming and bioengineering.

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    • Properties
      • Categories
        Processing of protein, nucleic acid and polypeptide purification
        Note
        (1)The sample and Ni seplife®6FF (NTA) must be equilibrated with the elution buffer and then go to chromatography column.
        (2)The column bed must be flat, free of grooves and air bubbles, otherwise it should be reinstalled.
        (3)When using, ensure that the temperature of the column and the buffer are the same, avoiding the generation of bubbles in the column bed and affecting the purification effect.
        (4)The flow rate should be strictly controlled during the elution process, and not too fast.
        (5)During the loading and the entire elution process, the cylinder surface is prevented from drying out.
    • Applications
      • Application Description
        Separation and Purification of Sugar and Alcohol

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