ioGlutamatergic Neurons have been reprogrammed from human induced pluripotent stem cells (iPSC) using our precise reprogramming technology: opti-ox™¹ (optimized inducible overexpression). Human stem cells, within days, convert into consistent, mature, functional glutamatergic neurons providing a high quality human model for the study of neurological activity and disease.
ioGlutamatergic Neurons consist mainly of glutamatergic neurons (>80%) characterised by the expression of the glutamate transporter genes VGLUT1 and VGLUT2. The minor remaining fraction of the neuronal population express marker genes of cholinergic neurons. A bulk RNA-seq analysis shows that ioGlutamatergic Neurons have a rostral CNS identity and express the classical cortical marker genes FOXG1 and TBR1.
Ready-to-culture cells are suitable as models for research in cell-type specific biology, target validation and drug screening in pharmaceutical R&D, and toxicology testing.
Batch to batch reproducibility and homogeneity create a stable human model for excitatory neuronal activity and disease.
Ready for experimentation as early as 2 days post revival and form functional neuronal networks at 17 days.
Industrial scale quantities at a price point that allows the cells to be used from research to screening scale.
■ Ease of Use
Cells arrive programmed to rapidly mature upon revival. One medium required in a two-step protocol.
Please contact us at for specific academic pricing.
TypeHuman-induced glutamatergic neuronsStorageUpon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments
* These cells are for research use only
Application Description– Academic research
– Drug development
– High-throughput screening
– Single cell CRISPR Screening
– 3D bioprinting
Zhou, L., Wolfes, A. C., Li, Y., Chan, D. C. W., Ko, H., Szele, F. G., Bayley, H., Lipid‐Bilayer‐Supported 3D Printing of Human Cerebral Cortex Cells Reveals Developmental Interactions. Adv. Mater. 2020, 32, 2002183.
Pawlowski, Matthias et al. Inducible and Deterministic Forward Programming of Human Pluripotent Stem Cells into Neurons, Skeletal Myocytes, and Oligodendrocytes. Stem Cell Reports, Volume 8, Issue 4, 803 - 812
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