Human Stem Cell Factor (AF)

Human Stem Cell Factor (AF)

Catalog Number:
P001418156ABE
Mfr. No.:
Abe32-12303
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      • Overview
        • Predicted MW: Monomer, 18.6 kDa (165 aa). Stem cell factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor c-Kit/CD117 and plays a critical role in the maintenance, survival, and differentiation of hematopoietic stem cells. While human SCF shows no activity on murine cells, murine and rat SCF are active on human cells.

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      • Properties
        • Protein Name
          Resistin
          Source
          Genetically modified E.coli
          Type
          Recombinant Proteins
          Purification
          Reducing and Non-Reducing SDS PAGE at >= 95%
          Formulation
          Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM acetic acid or New Formulation with Lot 0417-100-03 and 0417-100-03AF and moving forward: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5 (available by request until we run out of acetic acid formulation lots).
          Sterile 10 mM HCl at 0.1 mg/mL for acetic acid formulaiton or
          Sterile water at 0.1 mg/mL for PBS formulation.
          Storage
          Store at -20°C
          Endotoxin
          Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
          Activity
          Biological Activity was determined by TF-1 cell proliferation at = 6.6 x 10^4 units/mg . Centrifuge vial before opening, Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws. Upon reconstitution, a small amount of visible precipitate can be expected. A 10% overfill has been added to the total material vialed to compensate for this loss.

          More Information

          UniProt
          Gene ID
          4254

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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