Name: Human MYL2 antibody
Price: 592.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    mouse
    
    Antibody Type
    
    monoclonal
    
    Isotype
    
    IgG2b kappa
    
    Reactivity
    
    human
    
    Clone
    
    AT3B2
    
    Immunogen
    
    Recombinant human MYL2 (1-166aa) purified from E. coli
    
    Purification
    
    By protein-A affinity chromatography
    
    Formulation
    
    Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
    
    Storage
    
    Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
    
    Concentration
    
    1mg/ml (determined by BCA assay)
    
    Antigen
    
    Slow cardiac myosin regulatory light chain 2, MLC2, CMH10, DKFZp779C0562, Slow cardiac myosin regulatory light chain 2, MYL2, Slow cardiac myosin regulatory light chain 2 Cardiac myosin light chain-2, MLC 2v, MYL 2, Cardiac ventricular myosin light chain 2, RLC of myosin, Myosin light chain 2 regulatory cardiac slow, Myosin light polypeptide 2 regulatory cardiac slow, Myosin regulatory light chain 2 ventricular cardiac muscle isoform, Myosin regulatory light chain 2 ventricular/cardiac muscle isoform, Regulatory light chain of myosin (UniProt:P10916)
    
    * For research use only. Not intended for human, diagnostics or veterinary use.
- Applications
  - Application
    
    ELISA; WB; FACS
    
    Application Description
    
    The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Reference
  - Sapp V, et al. Genome-wide CRISPR/Cas9 screening in human iPS derived cardiomyocytes uncovers novel mediators of doxorubicin cardiotoxicity. (Sci Rep. 2021)

The tissue lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYL2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse Heart Tissue lysate

Human MYL2 antibody

Human MYL2 antibody

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