Name: Human MIF antibody
Price: 544.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    mouse
    
    Antibody Type
    
    monoclonal
    
    Isotype
    
    IgG1 kappa
    
    Reactivity
    
    human
    
    Clone
    
    4E4
    
    Immunogen
    
    Recombinant human MIF (1-115 aa) purified from E. coli
    
    Purification
    
    By protein-G affinity chromatography
    
    Formulation
    
    Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
    
    Storage
    
    Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
    
    Concentration
    
    1mg/ml (determined by BCA assay)
    
    Antigen
    
    Macrophage migration inhibitory factor, GLIF, MMIF, MIF, EC 5.3.2.1, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, Macrophage migration inhibitory factor, macrophage migration inhibitory factor (glycosylation-inhibiting factor), (UniProt:P14174)
    
    * For research use only. Not intended for human, diagnostics or veterinary use.
- Applications
  - Application
    
    ELISA; WB; ICC/IF
    
    Application Description
    
    The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Reference
  - Koizumi M, et al. Positive correlations of age and parity with plasma concentration of macrophage migration inhibitory factor in Japanese black cows. (J Reprod Dev. 2016)

ICC/IF analysis of MIF in Balb/3T3 cells. The cell was stained with AMF0608 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Human MIF antibody

Human MIF antibody

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