Name: Human HSP27 antibody
Price: 544.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    mouse
    
    Antibody Type
    
    monoclonal
    
    Isotype
    
    IgG1 kappa
    
    Reactivity
    
    human
    
    Clone
    
    2A5
    
    Immunogen
    
    Recombinant human Hsp27 (1-205aa) purified from E. coli
    
    Purification
    
    By protein-G affinity chromatography
    
    Formulation
    
    Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
    
    Storage
    
    Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
    
    Concentration
    
    1mg/ml (determined by BCA assay)
    
    Antigen
    
    Heat shock 27 kDa protein, Hspb1, Hsp25, Hsp27, Hsp27, HspB1, Heat shock 27 kDa protein, HSP 27, Growth-related 25 kDa protein, P25, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein 28 kDa heat shock protein, CMT2F, DKFZp586P1322, Estrogen regulated 24 kDa protein, at shock 25kDa protein 1, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat Shock Protein 27, Heat shock protein beta 1, Heat Shock Protein27, HS.76067, Hsp 28, Hsp B1, Hsp28, HspB1, SRP27, Stress responsive protein 27. (UniProt:P04792)
    
    * For research use only. Not intended for human, diagnostics or veterinary use.
- Applications
  - Application
    
    ELISA; WB; ICC/IF; IHC; FACS
    
    Application Description
    
    The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Reference
  - Kaiser F, et al. Association between circulating levels of heat-shock protein 27 and aggressive periodontitis. (Cell Stress Chaperones. 2018)
    Eickholz P, et al. Effect of nonsurgical periodontal therapy on haematological parameters in grades B and C periodontitis: an exploratory analysis. (Clin Oral Investig. 2020)

ICC/IF analysis of Hsp27 in HeLa cells. The cell was stained with AHS0702 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Human HSP27 antibody

Human HSP27 antibody

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