Name: Human alpha-Smooth Muscle Actin/ACTA2 antibody
Price: 544.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    mouse
    
    Antibody Type
    
    monoclonal
    
    Isotype
    
    IgG1 kappa
    
    Reactivity
    
    human
    
    Clone
    
    AT132D3
    
    Immunogen
    
    Recombinant human ACTA2 (3-377aa) purified from E. coli
    
    Purification
    
    By protein-A affinity chromatography
    
    Formulation
    
    Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
    
    Storage
    
    Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
    
    Concentration
    
    1mg/ml (determined by BCA assay)
    
    Antigen
    
    Aortic smooth muscle actin, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, Actin, Aortic smooth muscle, Intermediate form, ACTSA, ACTVS, GIG46 (UniProt:P62736)
    
    * For research use only. Not intended for human, diagnostics or veterinary use.
- Applications
  - Application
    
    ELISA; WB; ICC/IF; FACS
    
    Application Description
    
    The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Reference
  - Liu X, et al. Human aortic valve interstitial cells obtained from patients with aortic valve stenosis are vascular endothelial growth factor receptor 2 positive and contribute to ectopic calcification. (J Pharmacol Sci. 2021)

ICC/IF analysis of ACTA2 in HeLa cells. The cell was stained with ATGA0358 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Human alpha-Smooth Muscle Actin/ACTA2 antibody

Human alpha-Smooth Muscle Actin/ACTA2 antibody

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