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Overview
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GRXB produced in E. Coli is a single, non-glycosylated polypeptide chain containing 235 amino acids (1-215 a.a.) and having a molecular mass of 26.5kDa. GRXB is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Glutaredoxin-2 (GrxB) is amember of the glutaredoxin family. Glutaredoxins are small redox enzymes of approximately 100 amino-acid residues which use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. GrxB is involved in reducing some disulfides in a coupled system with glutathione reductase. GrxB doesn't act as hydrogen donor for ribonucleotide reductase.
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- Properties
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Overview