Login / Register

0

Figure 1. Detection of non-phosphorylated human GPR35 Receptor. Flp-In TREx 293 harbouring human GPR35-eYFP induced to express the receptor construct were treated with vehicle or 100 nM Lodoxamide for 5 min. Cells were lysed and receptor construct enriched with a GFP-trap, before immunoblotting with anti-non-phospho-GPR35 at a dilution of 1:1000 (upper panel) or anti-GFP 1:10,000 to confirm expression and equal loading of the gel (bottom panel).

Figure 2. Detection of non-phosphorylated mouse GPR35 Receptor. Flp-In TREx 293 harbouring mouse GPR35-HA induced to express the receptor construct were treated with vehicle or 10 µM Zaprinast for 5 min. Cells were lysed and receptor construct enriched with an HA-trap, before immunoblotting with anti-non-phospho-GPR35 at a dilution of 1:1000 (upper panel) or anti-HA 1:10,000 to confirm expression and equal loading of the gel (bottom panel).

Figure 3. Immunohistochemical identification of GPR35 Receptor in human colon. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR35 antibody (7TM0102N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, GPR35 receptors were detected at the plasma membrane of epithel cells in human colon and in macrophages.

Figure 4. Immunohistochemical identification of GPR35 Receptor in Crohn's disease. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR35 antibody (7TM0102N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, GPR35 receptors were detected at the plasma membrane of epithel cells in human colon and in macrophages.

Figure 5. Immunohistochemical identification of GPR35 Receptor in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR35 antibody (7TM0102N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, GPR35 receptors were detected in macrophages.

Figure 6. Immunohistochemical identification of GPR35 Receptor in gastric carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR35 antibody (7TM0102N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, GPR35 receptors were detected at the plasma membrane of tumor cells in human gastric carcinoma.

GPR35 ( non-phospho ), G Protein-coupled Receptor 35 Polyclonal Antibody

GPR35 ( non-phospho ), G Protein-coupled Receptor 35 Polyclonal Antibody

Catalog Number:

A001455914TMA

Mfr. No.:

7TM0102N

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The non-phospho-GPR35 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human GPR35. It can be used to detect total GPR35 receptors in Western blots independent of phosphorylation. The non-phospho-GPR35 antibody can also be used to detect GPR35 in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse
  
  Immunogen
  
  A synthetic peptide presents the carboxyl-terminal tail of human GPR35.
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  GPR35, G-protein coupled Receptor 35 (UniProt:Q9HC97 (human), Q9ES90 (mouse))
  
  * This product is intended for research use only.

Applications

Application

WB; IHC

Application	Dilutions
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100

Reference
- Divorty N, Jenkins L, Ganguly A, Butcher AJ, Hudson BD, Schulz S, Tobin AB, Nicklin SA, Milligan G. Agonist-induced phosphorylation of orthologues of the orphan receptor GPR35 functions as an activation sensor. J Biol Chem. 2022 Mar;298(3):101655. doi: 10.1016/j.jbc.2022.101655. Epub 2022 Jan 29. PMID: 35101446; PMCID: PMC8892012.

Note: If you don't receive our verification email, do the following:

Confirm that you entered your email address correctly.
Check if the email is in your spam or junk folder.
Or you may contact us at .

Products

Amerigo Scientific

Support

Contact Us

Copyright © Amerigo Scientific. All rights reserved.