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GCLM Recombinant Protein

GCLM Recombinant Protein

Catalog Number:
P001416583ABE
Mfr. No.:
Abe32-2354
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  • Size:
    20 µg
    Quantity:
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      • Overview

        • GCLM Human Recombinant produced in E. coli is a single polypeptide chain containing 298 amino acids (1-274) and having a molecular mass of 33.3kDa. GCLM is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Glutamate-cysteine ligase (GCLM) is the first rate limiting enzyme of glutathione synthesis. The GCLM enzyme is comprised of 2 subunits, a heavy catalytic subunit and a light regulatory subunit. GCLM deficiency is associated with some forms of hemolytic anemia.

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      • Properties
        • Protein Name
          Glutamate--cysteine ligase regulatory subunit, GCS light chain, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GCLM, GLCLR.
          Source
          E. coli
          Type
          Recombinant Proteins
          Purification
          Greater than 90.0% as determined by SDS-PAGE.
          Formulation
          The GCLM solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.15M NaCl.
          Storage
          Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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