Endonuclease, >250U/μL

Overview
- Our Endonuclease is a genetically engineered endonuclease.
  It cleaves efficiently all kinds of DNA and RNA into small fragments, irrespective of single-stranded, double-stranded, linear, circular or supercoiled. The enzyme can be used for various applications, such as removal of DNA/RNA from solutions, reduction of cell lysate viscosity caused by nucleic acids, and so on.
  
  Please contact us at for specific academic pricing.
Properties
- Source
  
  Originating from the microorganism Serratia marcescens, and expressed in Pichia pastoris.
  
  Purification
  
  Pruity: ≥99%
  
  Storage
  
  Our Endonuclease is rather stable, but storage under extreme conditions will lead to activity loss. The optimum storage temperature is -20°C.
  
  * For research use only.

Applications

Application	Dilutions
Our Endonuclease has high nuclease activity and is generally applied for removal of contaminant DNA and viscosity reduction.
E. coli cells were suspended in 1L of Tris-HCl buffer pH7.0 to obtain a suspension of OD600 = 100. 10mL of 1M MgCl2 was added. Our Endonuclease was added to obtain a final concentration of 100U/mL. The cells were lysed on ice with an ultrasonic prove for 30 minutes. The lysate was incubated at 37°C for 30 minutes. Following centrifugation at 12,000xg (15min., 25°C), cell lysate with low viscosity was obtained.

Application

Dilutions

Our Endonuclease has high nuclease activity and is generally applied for removal of contaminant DNA and viscosity reduction.

E. coli cells were suspended in 1L of Tris-HCl buffer pH7.0 to obtain a suspension of OD600 = 100. 10mL of 1M MgCl2 was added. Our Endonuclease was added to obtain a final concentration of 100U/mL. The cells were lysed on ice with an ultrasonic prove for 30 minutes. The lysate was incubated at 37°C for 30 minutes. Following centrifugation at 12,000xg (15min., 25°C), cell lysate with low viscosity was obtained.

Endonuclease, >250U/μL

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