EC50 glycan clean up plate

96-well plates

Binding Capacity: Each well of the LudgerClean™ EC50 plate can typically bind up to 50 μg of O- or N-linked glycans.
Number Of Samples: LudgerClean™ EC50 plates are designed for single use only.
Suitable Samples: A wide range of glycans can be purified. These include N-linked and O-linked type oligosaccharides, tri-saccharides and larger structures. The plate is not suitable either for monosaccharides or disaccharides which are generally bound too weakly for efficient purification or for large linear poly-sialylated glycans which can be bound very tightly to the resin. Glycan samples must be applied to the plate in solutions that are substantially aqueous.
Structural Integrity: No detectable (< 2 mole per cent) loss of sialic acid, fucose, sulfate, or phosphate.
Binding Efficiency: > 95 % for most glycans
Binding Selectivity: Essentially stoichiometric binding and elution for most complex glycan mixtures.
Handling: Ensure that any glass, plastic ware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.

Store at room temperature in the dark. Protect from sources of heat, light, and moisture. The plate is stable for at least two years as supplied.

The product can be shipped at ambient temperature.

For purification of glycans from a variety of complex mixtures (including removal of salts and detergents).