dLeptin Recombinant Protein

dLeptin Recombinant Protein

Catalog Number:
P001417875ABE
Mfr. No.:
Abe32-1565
Price:
  • Size:
    100 µg
    Quantity:
    Add to Cart:
      • Overview
        • Leptin Dog Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16 kDa. The Leptin is purified by proprietary chromatographic techniques. A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.

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      • Properties
        • Protein Name
          OB Protein, Obesity Protein, OBS, Obesity factor.
          Source
          E. coli
          Type
          Recombinant Proteins
          Purification
          Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
          Formulation
          The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
          Storage
          Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

      • Applications
        • Application Description
          It is recommended to reconstitute the lyophilized Leptin in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.

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