Name: DAG1 Recombinant Protein
Price: Inquiry

- Overview
  - DAG1 Human Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain (a.a 30-312) containing 293 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 31.87kDa (calculated). Dystroglycan 1 (DAG1) is a laminin binding component of the dystrophin-glycoprotein complex which provides a connection between the subsarcolemmal cytoskeleton and the extracellular matrix. The N-terminal domain of alpha-dystroglycan is secreted into the cerebrospinal fluid. The effect of DAG1 on the nervous system remains vague. The complete dystroglycan complex is expressed in a various tissues and has a role in processes such as laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. DAG1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic decrease of DAG1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, making muscle fibers more susceptible to necrosis.
    
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- Properties
  - Protein Name
    
    Dystroglycan, Dystrophin-associated glycoprotein 1, DAG1, A3a, DAG, AGRNR, 156DAG, MDDGC7, MDDGC9.
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    Greater than 90.0% as determined by SDS-PAGE.
    
    Formulation
    
    DAG1 filtered (0.4µm) and lyophilized from 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4.
    
    Storage
    
    Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.
- Applications
  - Application Description
    
    It is recommended to add 200µl deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. DAG1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

DAG1 Recombinant Protein

DAG1 Recombinant Protein

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