Name: CyFlow™ CD28 Low Endotoxin
Price: 324.00 USD

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express α/β and γ/δ TCR. The level of CD28 on mature CD4+ and CD8+ α/β TCR+ thymocytes is two- to fourfold lower than on the immature cells.
- Properties
  - Host
    
    hamster
    
    Antibody Type
    
    monoclonal
    
    Isotype
    
    IgG
    
    Reactivity
    
    mouse
    
    Clone
    
    37.51
    
    Immunogen
    
    Mouse T cell lymphoma EL-4 cells
    
    Conjugate
    
    Low Endotoxin
    
    Formulation
    
    The reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
    
    Storage
    
    Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
    
    Concentration
    
    1 mg/mL
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Tp44
    
    * Research use only
- Applications
  - Application
    
    Flow Cytometry; Functional Assays; Immunocytochemistry; IHC (frozen); Immunoprecipitation