Name: CyFlow™ CD25 PE-Cy7
Price: 536.00 USD

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    CD25 (IL2Rα, Tac) is a ligand-binding α subunit of interleukin 2 receptor (IL2R). Together with β and γ subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.
- Properties
  - Host
    
    mouse
    
    Antibody Type
    
    monoclonal
    
    Isotype
    
    IgG1
    
    Reactivity
    
    human
    
    Clone
    
    MEM-181
    
    Immunogen
    
    PHA-activated peripheral blood leucocytes
    
    Conjugate
    
    PE/Cyanine7
    
    Formulation
    
    The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
    
    Storage
    
    Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    IL2Rα , p55, TAC
    
    * Research use only
- Applications
  - Application
    
    Flow Cytometry
    
    Application Description
    
    The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
    
    Emission Maximum: 785 nm
    Excitation Maximum: 496 nm, 565 nm