Name: Chicken LDHB Recombinant Protein
Price: Inquiry

- Overview
  - The DNA encoding chicken LDH-B is cloned from cDNA library of chicken heart. Lactate dehydrogenase (LDH) is an enzyme(EC1.1.1.27) present in a wide variety of organisms, including plants and animals. A tetrameric enzyme that catalyses the interconversion of pyruvateand lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
    
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- Properties
  - Protein Name
    
    Lactate Dehydrogenase, LDH.
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
    
    Formulation
    
    The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate.
    
    Storage
    
    Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.
- Applications
  - Application Description
    
    It is recommended to reconstitute the lyophilized LDH in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. The specific activity was found to be 258 U/mg protein.

Chicken LDHB Recombinant Protein

Chicken LDHB Recombinant Protein

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