CCNB1 Recombinant Protein

CCNB1 Recombinant Protein

Catalog Number:
P001416424ABE
Mfr. No.:
Abe32-2953
Price:
  • Size:
    10 µg
    Quantity:
    Add to Cart:
      • Overview
        • CCNB1 Human Recombinant produced in E. coli is a single polypeptide chain containing 457 amino acids (1-433) and having a molecular mass of 50.9 kDa. CCNB1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis. CCNB1 creates a complex with p34(cdc2) to form the maturation-promoting factor (MPF). CCNB1 is vital for the control of the cell cycle at the G2/M (mitosis) transition. CCNB1 builds up steadily during the G2 and is immediately destroyed at mitosis. The 2 alternative transcripts produce a constitutively expressed transcript and a cell cycle-regulated transcript which is expressed predominantly during G2/M phase. These transcripts are a result of alternate transcription initiation sites.

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      • Properties
        • Protein Name
          G2/mitotic-specific cyclin-B1, cyclin B1, CCNB.
          Source
          E. coli
          Type
          Recombinant Proteins
          Purification
          Greater than 80% as determined by SDS-PAGE.
          Formulation
          The CCNB1 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 150mM NaCl and 10% glycerol.
          Storage
          Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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