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Figure 1. Validation of the Cholecystokinin Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Cholecystokinin Receptor 1 (CCK1) were lysed and immunoblotted with the anti-CCK1 antibody (7TM0076N) at a dilution of 1:1000.

Figure 2. Immunocytochemical identification of Cholecystokinin Receptor 1 in HEK293 cells. HEK293 cells stably expressing the Cholecystokinin Receptor 1 (CCK1) were either not exposed or exposed to 1 μM CCK-8 (Cholecystokinin-8) for 30 min and immunocytochemically stained with anti-CCK1 (Cholecystokinin Receptor 1) antibody (7TM0076N) at a dilution of 1:200. Note, CCK1 receptors were confined to the plasma membrane in untreated cells (0 min). CCK1 receptors were seen in perinuclear clusters of vesicles after 30 min CCK-8 exposure.

Figure 3. Immunohistochemical identification of Cholecystokinin Receptor 1 in gastric mucosa. Sections were dewaxed, microwaved in citric acid, and incubated with anti-CCK1 (Cholecystokinin Receptor 1) antibody (7TM0076N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, CCK1 receptors were uniformly detected at the plasma membrane of chief cell in the gastric mucosa.

Figure 4. Immunohistochemical identification of Cholecystokinin Receptor 1 in neuroendocrine tumor. Sections were dewaxed, microwaved in citric acid, and incubated with anti-CCK1 (Cholecystokinin Receptor 1) antibody (77TM0076N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, CCK1 receptors were detected predominantly at the level of plasma membrane in neuroendocrine tumor.

CCK1 ( non-phospho ), Cholecystokinin Receptor 1 Polyclonal Antibody

CCK1 ( non-phospho ), Cholecystokinin Receptor 1 Polyclonal Antibody

Catalog Number:

A001455964TMA

Mfr. No.:

7TM0076N

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The CCK1 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human Cholecystokinin Receptor 1 (previously called CCKA). It can be used to detect total CCK1 receptors in Western blots independent of phosphorylation. The CCK1 antibody can also be used to isolate and enrich CCK1 receptors from cell and tissue lysates. It also detects CCK1 in cultured cells and tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human
  
  Immunogen
  
  A synthetic peptide with sequence TGASLSRFSYSHMSASVPPQ corresponds to amino acids 409-428 of human CCK1
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  CCK1, CCKAR, Cholecystokinin Receptor 1, Cholecystokinin Receptor A (UniProt:P32238)
  
  * This product is intended for research use only.

Applications

Application

WB; ICC; IHC

Application	Dilutions
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100

Reference
- Schulz S, Röcken C, Mawrin C, Schulz S. Immunohistochemical localization of CCK1 cholecystokinin receptors in normal and neoplastic human tissues. J Clin Endocrinol Metab. 2005 Nov;90(11):6149-55. Epub 2005 Aug 16. PubMed PMID: 16105969.

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