Name: Bulk anti-Human CD5 antibody (UCHT-2) - Low Endotoxin
Price: 680.00 USD

- Overview
  - Antigen Distribution: The CD5 antigen is present on approximately 70% of normal peripheral blood lymphocytes and >90% of all T-lymphocytes. T-lymphocytes in the thymus stain weakly while peripheral T-cells stain strongly. Monocytes, granulocytes and NK cells are not stained.1 Anti-CD5 also reacts with a distinct subset of normal B-lymphocytes, occasional cells in B-lymphocyte areas of spleen and lymph node, and most Ig+ B-chronic lymphoblastic leukemia (CLL) cells. The CD5 antigen has also been shown to be present on some lymphomas.
    Specificity: Mouse Anti-Human CD5 (Clone UCHT-2) recognizes Human CD5. Anti-Human CD5 recognizes a human T-lymphocyte surface membrane antigen (Mr 67 kDa).
    Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
    Isotype Control: Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin [HKSP] (ICH2247)
    Endotoxin: <1.0 EU/mg as determined by the LAL method
    Aggregation: Aggregation level ≤ 5%
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Anti-Human CD5 can be used in research to study B-lymphocyte subsets or characterization of leukemias and lymphomas.
- Properties
  - Host
    
    mouse
    
    Isotype
    
    IgG1
    
    Reactivity
    
    human
    
    Clone
    
    UCHT-2
    
    Purification
    
    ≥95% monomer by analytical SEC, >95% by SDS Page
    
    Formulation
    
    This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
    
    Storage
    
    This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
    
    Molecular Weight
    
    ≥ 5.0 mg/ml
    
    Regulatory Status
    
    The suggested concentration for this CD5 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
    
    Antigen
    
    (Gene ID:Leu-1)
    
    * Products are for research use only. Not for use in diagnostic or therapeutic procedures.
- Applications
  - Application
    
    CODEX®; Functional Assays; Flow Cytometry; IHC (frozen)

Bulk anti-Human CD5 antibody (UCHT-2) - Low Endotoxin

Bulk anti-Human CD5 antibody (UCHT-2) - Low Endotoxin

Background

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