Name: Bulk anti-Human CD3 antibody (UCHT-1) - Low Endotoxin
Price: 680.00 USD

- Overview
  - Antigen Distribution: The CD3 antigen is expressed on approximately 60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and approximately 70% of T-ALL Cells.
    Specificity: Anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) recognizes Human CD3. anti-Human CD3 recognizes a (Mr 22-28 kDa) T-cell surface glycoprotein. The epitope recognized by the CD3 antibody is expressed on a constant region of the epsilon chain of the CD3 antigen/T-cell receptor complex (TCR)
    Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
    Isotype Control: Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin [HKSP] (ICH2247)
    Endotoxin: <1.0 EU/mg as determined by the LAL method
    Aggregation: Aggregation level ≤ 5%
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Anti-CD3 may be used for enumerating immunocompetent T-lymphocytes in peripheral blood. Clone UCHT-1 is also useful in histology for localization of T-lymphocytes in tissue and may be used to enrich T-cells by cell sorting. This T-cell marker has also been used to determine T-versus B-cell lymphomas and leukemias. The UCHT-1 clone is recognized in the human leukocyte differentiation antigen workshop III 471.
- Properties
  - Host
    
    mouse
    
    Isotype
    
    IgG1
    
    Reactivity
    
    human
    
    Clone
    
    UCHT-1
    
    Immunogen
    
    Unknown
    
    Purification
    
    >95% by SDS-PAGE and HPLC
    
    Formulation
    
    0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
    
    Storage
    
    anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
    
    Molecular Weight
    
    ≥ 2.0 mg/ml
    
    Regulatory Status
    
    Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD3. A suggested method would be to stain cells expressing CD3 with 2.0 µg per million cells in a total staining volume of 100 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin.
    Each investigator should determine their own optimal working dilution for specific applications.
    
    Antigen
    
    (Gene ID:CD3 epsilon chain, CD3E, T-cell surface antigen T3/Leu-4 epsilon chain)
    
    * Products are for research use only. Not for use in diagnostic or therapeutic procedures.
- Applications
  - Application
    
    Flow Cytometry; Immunoprecipitation; WB; IHC (frozen); CyTOF; Functional Assays

Bulk anti-Human CD3 antibody (UCHT-1) - Low Endotoxin

Bulk anti-Human CD3 antibody (UCHT-1) - Low Endotoxin

Background

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