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Overview
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The Biotinylation Kit contains the reagents for the labeling of antibodies and other proteins and the purification of the biotinylated product.
The coupling reaction takes place at primary amino groups on the target molecule using a N-Hydroxysuccinimide (NHS) ester-activated biotin, which form stable amide bonds. Proteins generally have a sufficient number of amino groups for coupling, both N-terminal and lysine residues. In this kit, Sulfo-NHS-LC Biotin (sulfosuccinimidyl-6-[biotin-amido] hexanoate) is used, that contains a spacer group with 6 carbon atoms and a length of 22.4 Å to increase the distance between the target molecule and the biotin groups.
In the kit are 10 portions of Sulfo-NHS-LC Biotin reagent. One portion is used for a antibody amount of 1-10 mg or a protein amount of 1-5 mg.
Easy to conduct
Reliable conjugation
Excellent in combination with Polystrept R and StreptavidinPlease contact us at for specific academic pricing.
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- Properties
- Applications
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Overview