beta Actin Rabbit Monoclonal Antibody

beta Actin Rabbit Monoclonal Antibody

Catalog Number:
A001453914ZEN
Mfr. No.:
Zen-R23613
Price:
  • Size:
    100μl
    Quantity:
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      • Overview
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          Background

          beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.

      • Properties
        • Host
          rabbit
          Antibody Type
          loading control antibodies
          Isotype
          IgG
          Reactivity
          human; mouse; rat
          Clone
          R09-4C9
          Immunogen
          A synthetic peptide of human beta Actin
          Purification
          Affinity Purified
          Conjugate
          Unconjugated
          Formulation
          50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
          Storage
          Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
          Molecular Weight
          Calculated MW: 42 kDa; Observed MW: 42 kDa
          Antigen
          (Gene ID:60; UniProt:P60709)

          * Research Use Only Product (RUO). Not for Diagnosis or Therapeutic Use.

      • Applications
        • Application
          WB; IHC-F; IHC-P; ICC/IF
        • Application Dilutions
          WB 1/5000-1/10000
          IHC 1/50-1/200
          IF 1/100-1/200

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