Name: Bcl-2 -BH1 Recombinant Protein
Price: Inquiry

- Overview
  - Bcl-2 Des BH1 domain (136-155 residues) Human Recombinant produced in E. Coli is a single, non-glycosylated polypeptide chain containg 198 amino acids 1-135 and 156-218. The Bcl-2 is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques. BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
    
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- Properties
  - Protein Name
    
    Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2.
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
    
    Formulation
    
    The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.
    
    Storage
    
    Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bcl-2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.
- Applications
  - Application Description
    
    Suspend Bcl-2 in 100µl of 0.5M Acetic acid, over night at 4°C. Dilute 10 fold into selected buffer system.BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.

Bcl-2 -BH1 Recombinant Protein

Bcl-2 -BH1 Recombinant Protein

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