Name: Atlas Pfu DNA Polymerase
Price: 250.00 USD

- Overview
  - In addition to 5´ to 3´ DNA polymerase activity, Atlas Pfu DNA Polymerase also possesses 3´ to 5´ exonuclease (proofreading) activity. Atlas Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, is even up to ten fold more accurate than normal Taq DNA polymerase. Consequently, Atlas Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis.
    
    Supplied with:
    Atlas 10x Pfu Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 100 mM KCl,
    100 mM (NH4)2SO4, 1% Triton X-100, 1 mg/ml BSA
    Atlas 10x Pfu Buffer with MgSO4: 200 mM Tris-HCl (pH 8.8 at 25°C), 100 mM KCl,
    100 mM (NH4)2SO4, 1% Triton X-100, 1 mg/ml BSA, 25 mM MgSO4
    Atlas 25 mM MgSO4
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    DNA Polymerases
    
    Concentration
    
    5 units/µl
    
    Other Properties
    
    Quality control: Free of detectable, non-specific nucleases.
    Unit definition: One unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.
    Source: Recombinant E. coli strain with cloned gene encoding Pyrococcus furiosus DNA polymerase.
    
    Storage
    
    20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol. Store at -20°C. Guaranteed stable for 12 months when properly stored.
    
    * This product is supplied for research use only.
- Applications
  - Application Description
    
    – Close to ten times more accurate than normal DNA polymerase.
    – Produces blunt-ended amplification products to be used for cloning.
    – Remains active even after incubating 90 minutes at 95°C.

Datasheet

MSDS

Atlas Pfu DNA Polymerase

Atlas Pfu DNA Polymerase

We Also Recommend

WizScript™ RT Master

WizScript™ RT FDmix

WizPure™ PCR 2X Master