Name: Atlas HotTaq DNA Polymerase
Price: 250.00 USD

- Overview
  - Atlas HotTaq DNA Polymerase catalyzes 5´ to 3´synthesis of DNA. The enzyme has been proved not having the 3´ to 5´ exonuclease activity. Prior the first PCR step the Atlas HotTaq DNA Polymerase should be activated by 15 minute incubation at 95-97°C.
    
    Supplied with:
    Atlas 10x Taq Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% Nonident P40.
    Atlas 10x Taq Buffer with (NH4)2SO4: 750 mM Tris-HCl (pH 8.8 at 25°C),
    200 mM (NH4)2SO4, 0.1% Tween 20.
    Atlas 25 mM MgCl2
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    DNA Polymerases
    
    Concentration
    
    10 units/µl
    
    Other Properties
    
    Quality control: Free of detectable, non-specific nucleases. Activity and stability tested at 20, 30 and 40 cycles of PCR reaction at 95°C. Tested for the absence of human DNA contamination by PCR with Alu-specific primers.
    Unit definition: One unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.
    Source: Recombinant E. coli strain with cloned gene encoding Thermus aquaticus DNA polymerase.
    
    Storage
    
    20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol. Store at -20°C. Guaranteed stable for 12 months when properly stored.
    
    * This product is supplied for research use only.
- Applications
  - Application Description
    
    – Polymerase chain reaction setup at room temperature.
    – Effective incorporation of modified nucleotides.

Datasheet

MSDS

Atlas HotTaq DNA Polymerase

Atlas HotTaq DNA Polymerase

We Also Recommend

WizScript™ RT Master

WizScript™ RT FDmix

WizPure™ PCR 2X Master