Name: Anti-PKC antibody
Price: 224.00 USD

Overview
- PKC is a protein encoded by the PRKCA gene which is approximately 76, 7 kDa. PKC is localised to the cytoplasm and cell membrane. It is involved in RET signalling, DAG and IP3 signalling, the IL-2 Pathway, fMLP pathway and CDK-mediated phosphorylation and removal of Cdc6. It is a family of serine-and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. It plays a role in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. PKC is expressed in the liver, blood, nervous system, skin and pancreas. Mutations in the PRKCA gene may result in glioma and myotonic dystrophy. STJ95117 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of PKC protein.
  
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Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  Synthesized peptide derived from human PKC around the non-phosphorylation site of T497.
  
  Purification
  
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Concentration
  
  1 mg/ml
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Protein Kinase C Alpha Type; Pkc-A; Pkc-Alpha (Gene ID:5578; UniProt:P17252)
  
  * For research use only

Applications

Application

WB; IHC (paraffin); IF; ELISA

Application	Dilutions
WB	1:500-1:2000
IHC	1:100-1:300
IF	1:200-1:1000
ELISA	1:5000

Immunohistochemical analysis of paraffin-embedded Human Right kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PKC-pan Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human Right kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunofluorescence analysis of HUVEC cells, using PKC-pan Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from NIH/3T3 cells, treated with PMA 250ng/ml 15', using PKC-pan Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of Hela cell. 1, PKC Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-PKC antibody

Anti-PKC antibody

Immunohistochemical analysis of paraffin-embedded Human Right kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PKC-pan Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human Right kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunofluorescence analysis of HUVEC cells, using PKC-pan Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from NIH/3T3 cells, treated with PMA 250ng/ml 15', using PKC-pan Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of Hela cell. 1, PKC Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-PKC antibody

Anti-PKC antibody

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