Name: Anti-Phospho-NFKBIA-Tyr-42 antibody
Price: 596.00 USD

Overview
- This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-I Kappa B Alpha (Tyr-42) peptide (without carrier). The antibody detects a 38 kDa* protein on SDS-PAGE immunoblots of A431 and Jurkat cells treated with pervanadate, but not in control cells. Due to homologies to tyrosine sites on other proteins, it is recommended that the antibody be used to detect phosphorylation of immunoprecipitated I Kappa B Alpha.
  
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Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  I Kappa B Alpha (Tyr-42) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 42 of human I Kappa B Alpha. This peptide sequence has low homology to other I Kappa B proteins, but does have some homology to unrelated proteins that may have a conserved tyrosine phosphorylation motif.
  
  Purification
  
  Antigen Affinity Purified
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
  
  Storage
  
  Store at -20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
  
  Antigen
  
  (UniProt:P25963)
  
  * For Research Use Only (RUO). Not Used in Diagnostic or Therapeutic Applications.

Applications

Application	Dilutions
WB	1:1000
IP	1:100

Anti-Phospho-NFKBIA-Tyr-42 antibody