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Western blot analysis of extracts of HeLa cells, using Phospho-IKKγ-S376 pAb (STJ11101145) at 1:1000 dilution or IKKγ antibody (STJ24154).HeLa cells were treated by TNF-α (20 ng/mL) at 37°C for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using Phospho-IKKγ-S376 antibody (STJ11101145) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by TNF-α (20 ng/mL) at 37°C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.

Anti-Phospho-IKBKG-S376 antibody

Anti-Phospho-IKBKG-S376 antibody

Catalog Number:

A001018837STJ

Mfr. No.:

STJ11101145

Price:

$295

Size:

Quantity:

Add to Cart:

Overview
- Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  A phospho specific peptide corresponding to residues surrounding S376 of human IKBKG.
  
  Purification
  
  Affinity purification
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  
  Storage
  
  Store at -20°C and avoid freeze-thaw cycles.
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Nf-Kappa-B Essential Modulator; Nemo; Fip-3; Ikb Kinase-Associated Protein 1; Ikkap1; Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Gamma; I-Kappa-B Kinase Subunit Gamma; Ikk-Gamma; Ikkg; Ikb Kinase Subunit Gamma; Nf-Kappa-B Essential Modifier (Gene ID:8517; UniProt:Q9Y6K9)
  
  * For research use only

Applications

Application

WB

Application	Dilutions
WB	1:500-1:2000

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