Name: Anti-Mouse CD40 In Vivo Antibody - Low Endotoxin (FGK4.5)
Price: 680.00 USD

- Overview
  - Antigen Distribution: B cells, basal epithelial cells, macrophages, follicular dendritic cells, and endothelial cells
    Specificity: Anti-CD40 In Vivo Antibody - Low Endotoxin (FGK4.5) recognizes an epitope on Mouse CD40
    Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
    Isotype Control: Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)
    Endotoxin: <0.5 EU/mg as determined by the LAL method
    Aggregation: Aggregation level ≤ 5%
    
    Please contact us at for specific academic pricing.
    
    Background
    
    CD40, also known as TNFSF5, is a type I transmembrane protein and member of the TNF receptor family. The binding of CD40L (CD40) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 is expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40L is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. Some of the early intracellular signaling by the CD4-CD40L system includes the association of the CD40 with TRAFs and the activation of various kinases (4). Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of CD4-CD40L is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
- Properties
  - Host
    
    rat
    
    Isotype
    
    IgG2a
    
    Reactivity
    
    mouse
    
    Clone
    
    FGK4.5
    
    Immunogen
    
    Recombinant Mouse CD40 Fusion Protein
    
    Purification
    
    >95% by SDS-PAGE and HPLC
    
    Formulation
    
    0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added
    
    Storage
    
    anti-CD40 In Vivo Antibody - Low Endotoxin (FGK4.5) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
    
    Molecular Weight
    
    1.0 - 5.0 mg/ml
    
    Regulatory Status
    
    Each investigator should determine their own optimal working dilution for specific applications.
    
    * Products are for research use only. Not for use in diagnostic or therapeutic procedures.
- Applications
  - Application
    
    WB; Functional Assays; Flow Cytometry

Anti-Mouse CD40 In Vivo Antibody - Low Endotoxin (FGK4.5)

Anti-Mouse CD40 In Vivo Antibody - Low Endotoxin (FGK4.5)

Background

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