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Overview
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Beta actin is a cytoskeletal protein of the actin family, which is approximately 41, 7 kDa and localised to the cytoplasm. It is encoded by the ACTB gene and is involved in pathways such as blood-brain barrier and immune cell transmigration, regulation of actin cytoskeleton by Rho GTPases and development Slit-Robo signalling. Beta actin is one of 3 main groups of actin isoforms in vertebrates namely for which alpha and gamma actins are the other two. Beta actin is a highly conserved proteins that is involved in various types of internal cell motility and are the major constituent of microfilaments and it is ubiquitously expressed in all eukaryotic cells. Mutations in the ACTB gene can result in a form of juvenile-onset dystonia, which is defined by the presence of sustained involuntary muscle contraction-often leading to abnormal postures. Another disease, Baraitser-Winter syndrome 1, is also caused by ACTB mutation. This is rare developmental disorder that causes postnatal short stature and microcephaly, intellectual disability, seizures, and hearing loss. STJ96930 was developed to target endogenous beta actin, and is affinity-purified from mouse ascites by affinity-chromatography.
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Overview
Western blot analysis of Hela (1) Rat brain (2) Mouse brain (3) , Chicken lung (4) and Rabbit testis (5), diluted at 1:10000.
IHC Staining of Human ovary tissue, diluted at 1:200 Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, Beta-actin monoclonal antibody (5B7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1, Beta-actin monoclonal antibody (5B7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, Beta-actin monoclonal antibody (5B7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary antibody was diluted at 1:5000, 4°C over night, secondary antibody (cat： (NA was diluted at 1:10000, 37°C 1hour.
Immunofluorescence analysis of Hela cell. 1, Cyclin A Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-actin monoclonal antibody (5B7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).
Anti-Beta-actin antibody
Anti-Beta-actin antibody
Catalog Number:
A001052299STJ
Mfr. No.:
STJ96930
Price:
$224
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