-
-
Overview
-
• mouse monoclonal
• AFDyeTM 647 conjugate
• suitable for western blot analysis
• reacts with free VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
• isotype: IgG1Please contact us at for specific academic pricing.
Background
The B1 antibody reacts with free VP1, VP2 and VP3 of adeno-associated virus (AAV) and at a reduced degree with assembled viral particles. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus et al., 2000) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.
Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).
The AFDye™ 647 is a bright, far-red-fluorescent dye ideally suited for the 633 nm or 647 nm laser lines (excitation/emission 648/671 nm). It is structurally similar to Alexa Fluor® 647, and can be used with a common Cy5 filter set. A significant advantage to using long wavelength dyes such as Cy5 or AFDye™ 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.
Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.
-
- Properties
- Applications
-
Overview