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Overview
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Recombinant ACKA produced in E.coli is a single, non-glycosylated polypeptide chain containing 423 amino acids (1-400) and having a molecular mass of 45.7 kDa. The ACKA is fused to a 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. E. ColiAcetate Kinase, also knowns as ACKA, catalyzes the formation of acetylphosphate from acetate and ATP and also catalyzes the reverse reaction. ACKAtakes part in synthesis of various ATP formed catabolically during anaerobicgrowth of the organism.
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Overview