3D Organoid and Cellular Microenvironment Preparation and High-Resolution Detection System

In vivo, the cellular microenvironment has a crucial impact on the regulation of cell behavior and functions such as cellular differentiation, proliferation and migration. However, its biochemical and structural complexity limits our understanding of the key parameters regulating cell behavior.
In vitro, one of the challenges confronting cell biologists is therefore to work with controlled and reproducible microenvironments to more efficiently study living cells and model diseases.

Studying the influence of the microenvironment on intracellular and intercellular mechanisms has been essential for research in cell biology, for many years now. But in this quest, in vitro cell experiments confront researchers with many challenges, such as:
• the recurring reproducibility issues,
• reliability in term of physiological relevance,
• but also ease of use and efficiency.

This is why our research team has developed PRIMO: a photopatterning system performing micropatterning, microfabrication and hydrogel polymerization to overcome all this issues. We make it evolves to a new generation, PRIMO 2, with compact footprint, optic schematics allowing fluorescence and faster performances.

After preparation of the organoid and other cell models, the system can also detect them with high resolution (120nm after deconvolution)3D confocal imaging, suitable for long-term observation.

Product Principle
The system can create and prepare the organoid, organoid chip and other cell models through Micropatterning, Structuration of Hydrogel, Microfabrication and other mixed methods, and observe and detect them with high resolution (120nm after deconvolution)3D confocal imaging.

Product Characteristics
· Time saving & independence--Rapidly optimize your experimental conditions yourself.
· High Flexibility--Download any images you want to structure and/or functionalize your substrate.
· Versatility--Use your regular cell culture substrates: flat or microstructured, stiff or soft.
· Protein micropatterning: glass coverslips, glass slides, 96 well plates, EM grids, polystyrene, PDMS, polyacrylamide gel (transfer), hydrogels.
· Hydrogel structuration: UV-curable hydrogels, PEG Acrylate, Polyacrylamide, Agar, Matrigel.
· Microfabrication: UV-curable materials, UV-photoresists.
· More than 10 proteins used daily by our users, including: Fibrinogen-488, Fibrinogen-647, Fibronectin, GFP, Neutravidin-488, Neutravidin-647, PLL-PEG-Biotin, Protein A-647, Streptavidin, as well as primary and secondary antibodies.
· The photopatterning resolution is 1.2µm.
· The system can achieve multi-gradients etching with 256 gray levels.
· The fastest etching time for a full field pattern of 500×300µm with 20× objective is 0.5s.
· High-resolution 3D confocal imaging with resolution of 120nm after deconvolution.
· Higher sensitivity with increased SNR for 4 times, can detect weak fluorescence signal.
· Suitable for long-term live cell observation with nano-watt laser level on samples.
· Imaging speed: 2 fps for images of 512×512 pixels, the fastest speed is 10 fps.

Please contact us at for specific academic pricing.