3D Organoid and Cellular Microenvironment Preparation and High-resolution AI-analyzed Detection System

In vivo, the cellular microenvironment has a crucial impact on the regulation of cell behavior and functions such as cellular differentiation, proliferation and migration. However, its biochemical and structural complexity limits our understanding of the key parameters regulating cell behavior.
In vitro, one of the challenges confronting cell biologists is therefore to work with controlled and reproducible microenvironments to more efficiently study living cells and model diseases.

Studying the influence of the microenvironment on intracellular and intercellular mechanisms has been essential for research in cell biology, for many years now. But in this quest, in vitro cell experiments confront researchers with many challenges, such as:
• the recurring reproducibility issues,
• reliability in term of physiological relevance,
• but also ease of use and efficiency.

This is why our research team has developed PRIMO: a photopatterning system performing micropatterning, microfabrication and hydrogel polymerization to overcome all this issues. We make it evolves to a new generation, PRIMO 2, with compact footprint, optic schematics allowing fluorescence and faster performances.

After preparation of the organoid and other cell models, the system can also detect them with high-resolution (120nm) and AI-analyzed 3D confocal imaging, suitable for long-term observation.

Product Principle
The system can create and prepare the organoid, organoid chip and other cell models through Micropatterning, Structuration of Hydrogel, Microfabrication and other mixed methods, and observe and detect them with high resolution (120nm) and AI-analyzed 3D confocal imaging.

Product Characteristics
· Time saving & independence--Rapidly optimize your experimental conditions yourself.
· High Flexibility--Download any images you want to structure and/or functionalize your substrate.
· Versatility--Use your regular cell culture substrates: flat or microstructured, stiff or soft.
· Protein micropatterning: glass coverslips, glass slides, 96 well plates, EM grids, polystyrene, PDMS, polyacrylamide gel (transfer), hydrogels.
· Hydrogel structuration: UV-curable hydrogels, PEG Acrylate, Polyacrylamide, Agar, Matrigel.
· Microfabrication: UV-curable materials, UV-photoresists.
· More than 10 proteins used daily by our users, including: Fibrinogen-488, Fibrinogen-647, Fibronectin, GFP, Neutravidin-488, Neutravidin-647, PLL-PEG-Biotin, Protein A-647, Streptavidin, as well as primary and secondary antibodies.
· The photopatterning resolution is 1.2µm.
· The system can achieve multi-gradients etching with 256 gray levels.
· The fastest etching time for a full field pattern of 500×300µm with 20× objective is 0.5s.
· Large FOV (≥22mm) for imaging and detection.
· High scanning speed (FOV=22mm): 512×512 pixel≥30 fps; 1024×1024 pixel≥15 fps; 2048×2048 pixel≥7.5 fps.

Ultra-high resolution module: supports the improvement of the current image resolution under each objective lens, and supports the XY plane resolution ≤120nm and Z-axis resolution ≤300nm for the objective lens with a numerical aperture above 1.40.
Support 6-dimensional images and free combination, as: X/Y/Z dimensions, time, multi-wavelength and multi-point (including multi-point image stitching).
AI (artificial intelligence) module: including AI noise reduction module, after pre-training, can directly process the image online or offline, improve the image signal-to-noise ratio; Including AI separation module, automatic image analysis and target separation under AI analysis, for organoid samples in addition to fluorescent channels, also supports target separation on bright field images; The AI conversion module can train the image recognition without fluorescent label for pictures or long time series, and then carry out cell separation and target recognition analysis; The AI enhancement module can enhance image quality even after deep scanning of thick samples such as organoids after being intelligent trained for weaker images.
Suitable for long-term live cell observation.

Please contact us at for specific academic pricing.