In vivo | Collagen Hybridizing Peptides, sulfo-Cy7.5 Conjugates (sCy7.5-CHP)

The sCy7.5-CHPs are labeled with sulfonated-cyanine 7.5 dye for near-infrared fluorescence (NIRF) detection. This wavelength offers better tissue penetration while minimizing tissue autofluorescence.

Features
• New sequence designed for in vivo use- does not need any pre-activation step unlike CHPs for histology
• Near-infrared imaging probe minimizes the chance of autofluorescence in the gut or certain tissues
• Can be used for systemic or in situ injection
• Signal lasts at target sites for days, allowing for imaging at multiple time points with a single dose
• High affinity and unparalleled specificity to collagen turnover with essentially no nonspecific binding
• Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding
• Stable as powder form, stored at -20 °C

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Background

The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo.[1,2,3] By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.

The in vivo CHP incorporates our newest sequence which allows them to be directly injected into animals without having to pre-activate them with a heating step. [4] Therefore, the in vivo CHPs will always be in their active form and will not self-hybridize once injected. [4] The sCy7.5-CHP is a powerful in vivo tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging.[3] CHP can measure and localize mechanical injury to collagenous tissue at the molecular level.[5]

Specificity: CHP binds to the unfolded triple-helical chains of all collagen types (e.g., I, II, III, IV, etc).[3,6]