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Overview
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Bovine Serum Albumin (BSA) also referred as Fraction V (as it has been originally found in the fifth ethanol fraction using Cohn′s cold ethanol precipitation method) is a Serum Albumin Protein derived from bovine serum with a molecular weight of 66.4kDa. It is a water-soluble protein which consists of 583 amino acids. It consists of three homologous domains which have been formed by six α-helices.Albumin is the most abundant protein of the serum (about 60% of the total protein content of plasma). which regulates the osmotic pressure and pH of blood, and transports a wide variety of endogenous and exogenous compounds such as fatty acids, metal, amino acids, steroids and drugs. BSA, low immunoglobulin level, is a highly purified (more than 98%), lyophilized powder and can be used as a blocking reagent in EIA, RIA and ELISA systems, whereIgG can interfere with the application.
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