Login / Register

0

Figure 1. Agonist-induced Serine355/Serine360 phosphorylation of the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7. Upper panel, HEK293 cells stably expressing the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 (ACKR3/CXCR7) were either not exposed or exposed to 100 nM CXCL12 or 0.1 μM VUF 11207 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS355/pS360-ACKR3/CXCR7 antibody (7TM0080B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-ACKR3/CXCR7 antibody (7TM0080N) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Agonist-induced Serine350/Threonine352 phosphorylation of the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7. Upper panel, HEK293 cells stably expressing the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 (ACKR3/CXCR7) were either not exposed or exposed to 100 nM CXCL12 or 0.1 μM 0.1 μM VUF 11207 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS350/pT352-ACKR3/CXCR7 antibody (7TM0080A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-ACKR3/CXCR7 antibody (7TM0080N) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 3. Validation of the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 (ACKR3) were lysed and immunoblotted with the phosphorylation-independent c-terminal anti-ACKR3/CXCR7 antibody (7TM0080N) at a dilution of 1:1000. Note, ACKR3/CXCR7 forms stable dimers and multimers.

Figure 4. Validation of the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 (ACKR3) were lysed and immunoblotted with the phosphorylation-independent n-terminal anti-ACKR3/CXCR7 antibody (7TM0080N2) at a dilution of 1:1000. Note, ACKR3/CXCR7 forms stable dimers and multimers.

ACKR3 Sample Pack ( phospho- and non-phospho-Atypical Chemokine Receptor 3 Antibodies )

ACKR3 Sample Pack ( phospho- and non-phospho-Atypical Chemokine Receptor 3 Antibodies )

Catalog Number:

A001455908TMA

Mfr. No.:

7TM0080-SP

Price:

$464

Size:

4 x 20 µL

Quantity:

Add to Cart:

Overview
- ACKR3 Sample Pack consisting of all four available phospho- and non-phospho-Atypical Chemokine Receptor 3 Antibodies 4 x 20 µL trial size each. Specifically, this sample pack contains the following antibodies pS350/pT352-ACKR3 (7TM0080A), pS355/pS360-ACKR3 (7TM0080B), ACKR3 (non-phos, C-Term) (7TM0080N) and ACKR3 (non-phos, N-Term) (7TM0080N2).
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse
  
  Immunogen
  
  A synthetic phospho- and non-phosphopeptides derived from human ACKR3.
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  ACKR3, CXCR7, Atypical Chemokine Receptor 3, CXC Chemokine Receptor 7 (UniProt:P25106 (human), P56485 (mouse))
  
  * This product is intended for research use only.

Applications

Application

WB

Application	Dilutions
Western Blot (WB)	1:1000

Reference
- Saaber F, Schütz D, Miess E, Abe P, Desikan S, Ashok Kumar P, Balk S, Huang K, Beaulieu JM, Schulz S, Stumm R. ACKR3 Regulation of Neuronal Migration Requires ACKR3 Phosphorylation, but Not β-Arrestin. Cell Rep. 2019 Feb 5;26(6):1473-1488.e9. doi: 10.1016/j.celrep.2019.01.049.
  Sánchez-Alcañiz JA, Haege S, Mueller W, Pla R, Mackay F, Schulz S, López-Bendito G, Stumm R, Marín O. Cxcr7 controls neuronal migration by regulating chemokine responsiveness. Neuron. 2011 Jan 13;69(1):77-90. doi: 10.1016/j.neuron.2010.12.006.
  Hoffmann F, Müller W, Schütz D, Penfold ME, Wong YH, Schulz S, Stumm R. Rapid uptake and degradation of CXCL12 depend on CXCR7 carboxyl-terminal serine/threonine residues. J Biol Chem. 2012 Aug 17;287(34):28362-77. doi: 10.1074/jbc.M111.335679. Epub 2012 Jun 26. PubMed PMID: 22736769; PubMed Central PMCID: PMC3436560.

Note: If you don't receive our verification email, do the following:

Confirm that you entered your email address correctly.
Check if the email is in your spam or junk folder.
Or you may contact us at .

Products

Amerigo Scientific

Support

Contact Us

Copyright © Amerigo Scientific. All rights reserved.