Flow cytometry is a technique that can rapidly measure the biological properties of individual cells or organelles in a liquid flow system, and classify and collect specific cells or organelles from a population. Antibodies are essential reagents in flow cytometry because they allow for specific detection and quantification of cellular proteins and other molecules. Amerigo Scientific offers a range of flow cytometry antibodies for research purposes in a variety of fields. These antibodies are rigorously validated to ensure that the specific antibody is suitable for your intended assay or purpose.
Flow cytometry antibodies are monoclonal or polyclonal antibodies conjugated to fluorescent dyes. These antibodies specifically bind to target antigens on or within cells, allowing for the detection and analysis of these targets when excited by a laser in a flow cytometer.
Selecting the right antibodies and optimizing your staining protocol are key to obtaining reliable and meaningful data from your flow cytometry experiments.
These are antibodies that directly bind to the antigen of interest.
These antibodies bind to the primary antibody. They are usually conjugated with a fluorescent dye and are used when the primary antibody is unconjugated.
Flow cytometry antibodies have several advantages that make them valuable tools in a variety of biological and medical research applications.
| Specificity | Conjugation | Affinity | Validation | Species Reactivity |
| Select the antibody that binds specifically to the antigen of interest without cross-reactivity. | Choose an antibody conjugated with a fluorescent dye that matches the available laser lines and detection filters of your flow cytometer. | Choose high-affinity antibodies that provide better sensitivity and more accurate detection. | Ensure the antibody is validated for use in flow cytometry, as some antibodies may work well in other applications but not in flow cytometry. | Ensure the antibody is reactive to the species from which your sample is derived (e.g. human, mouse, rat). |
| Immunophenotyping | Identifying and quantifying different cell populations based on surface and intracellular markers (e.g., CD4, CD8, CD19). |
| Cell Cycle Analysis | Using antibodies against proteins like Ki-67 or BrdU to study cell proliferation. |
| Apoptosis Detection | Using antibodies against annexin V, cleaved caspase-3, or other apoptosis markers. |
| Intracellular Cytokine Staining | Detecting cytokines within cells after stimulation (e.g. IL-2, IFN-γ). |
| Functional Assays | Using phospho-specific antibodies to measure activation or inhibition of signaling pathways. |
Elevate your flow cytometry experience with our high-quality antibodies and trusted support for accurate proteolysis. Select excellence, select accuracy, and select Amerigo Scientific to meet your protein testing needs. Please for seamless flow cytometry success.
