Surface Plasmon Resonance (SPR) is a label-free optical technique used to study biomolecular interactions in real-time. It works by detecting changes in the refractive index of a surface-bound layer of molecules, typically proteins, as analyte molecules bind to them. This binding event causes a shift in the resonance angle of incident light, which is monitored and quantified to determine binding kinetics, affinity, and specificity. SPR antibodies are specifically designed and validated for use in SPR experiments, and serve as capture or detection molecules to target specific antigens or analytes to study protein-protein interactions, receptor-ligand binding, and other biomolecular interactions. Amerigo Scientific provides prominent antibody products used in SPR including monoclonal and polyclonal antibodies, as well as recombinant antibody fragments, which are tailored and strictly quality-controlled for reliable and accurate SPR experiments.
Fig.1 Schematic of the custom-made SPR setup (with the SPR curve and the resonance angle change).1
Our SPR antibodies are rigorously validated to ensure specific binding to the target analyte, minimizing non-specific interactions and enhancing the accuracy of SPR data.
With our SPR antibodies, researchers can detect even low concentrations of target molecules with detection limits in the picomolar range, enabling the study of weak interactions and providing valuable insights into biomolecular binding events.
Our antibodies demonstrate excellent batch-to-batch consistency and high stability, ensuring reliable and reproducible results across multiple experimental runs.
Our SPR antibody products facilitate the simultaneous screening of multiple samples, increasing efficiency and accelerating the discovery of potential drug candidates and biomolecular interactions.
SPR antibodies have diverse experimental applications across various research fields due to their ability to study biomolecular interactions in real-time. These antibodies are widely used in drug discovery to investigate molecular interactions between two or more molecules, enabling the screening of potential drug candidates and the characterization of drug-target interactions. In the field of molecular biology, SPR antibodies play a crucial role in studying protein-DNA and protein-RNA interactions, as well as antibody-antigen binding kinetics. Additionally, researchers in immunology utilize SPR antibodies to explore immune responses, epitope mapping, and antibody specificity. Furthermore, SPR antibodies find applications in the study of enzyme kinetics, signal transduction pathways, and biosensor development. Overall, the versatility of SPR antibodies makes them invaluable tools for researchers in drug discovery, molecular biology, immunology, and other fields, facilitating the investigation of a wide range of biomolecular interactions.
Fig.2 SPR kinetic analysis of the interaction between G4 nucleic acids and an anti-G4 monoclonal antibody.2
At Amerigo Scientific, high-quality and reliable SPR antibody products ensure that you get unparalleled SPR services and excellent results. In addition, we have the industry's high-standard platforms and instruments to help you achieve high-throughput and multifunctional analysis, as well as experienced experts to provide you with deeper insights and customized optimization. to explore our range of trustworthy SPR antibodies.
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