Western Blot is a common technique for identifying and assessing particular proteins in complex protein mixtures. In this method, proteins are separated by gel electrophoresis, transferred to a membrane, and then probed using particular antibodies binding to the target proteins. The bound antibodies are visualized using detection methods such as colorimetric, chemiluminescence, or fluorescence. Amerigo Scientific provides Western Blot antibodies for multiple research purposes. And at Amerigo Scientific, you can choose primary antibodies and secondary antibodies or polyclonal, monoclonal Western Blot antibodies in a variety of reactive species, including human, mouse, rat, canine, and cynomolgus. These products have been rigorously validated to ensure that the specific antibody is suitable for your intended detection or purpose.
Fig.1 Detection in Western Blots.
Western Blot antibodies are designed to recognize and bind to specific protein targets, enabling the accurate detection of the protein of interest amidst complex cellular mixtures. The specificity of the antibody has been verified by control tests (positive control, negative control), gene verification (gene knockout technology removal verification test), multiple-epitope approaches, and IP/MS.
Western blot antibodies enable consistent and reliable results across experiments, ensuring that data can be confidently replicated and compared. The experimental replication and biological reproducibility of antibodies have been verified. In addition, antibodies will use multiplexing, expression tags, and protein modification detection according to specific needs to further improve the consistency of results.
Western Blot antibodies are versatile tools with broad applications across various experimental, including IHC, IP, FACS, and IF tests. They can be used for protein detection and analysis, including biomarker discovery, post-translational modification analysis, protein expression profiling, and protein-protein interaction studies. In research fields such as immune checkpoint, stem cell research, drug target, and immunotherapy, Western Blot antibodies play a critical role in elucidating cellular processes, understanding disease mechanisms, and identifying potential therapeutic targets.
When selecting Western Blot antibodies, considering the endogenous target expression level is crucial. Understanding the natural abundance of the target protein in the sample can guide the choice of antibody sensitivity and detection range. Additionally, determining the appropriate antibody dilution and concentration is essential for optimal signal-to-noise ratio, thereby avoiding background interference and ensuring accurate quantification. Moreover, choosing the right blocking buffer is vital to minimize non-specific binding and enhance antibody specificity, with common choices including non-fat milk, BSA, or commercial blocking reagents. Amerigo Scientific provides systematic testing for Western Blot antibodies standard selection to help customers confirm and improve selectivity, ensuring the specificity and sensitivity of their Western Blot experiments, leading to more accurate and reproducible data.
Fig.2 Key elements of antibody validation.1
WB Protocols
Elevate your Western Blot experiments for accurate protein analysis with our high-quality antibodies and trustworthy support. Choose excellence, choose accuracy, and choose Amerigo Scientific for your protein detection needs. for seamless Western Blot success.
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