Immunohistochemistry (IHC) is an antibody-based technique used to detect protein expression in structures and tissues that have been retained. As one of the world's leading suppliers of immunohistochemistry antibodies, Amerigo Scientific offers extensively tested IHC immunohistochemistry antibodies. These antibodies have been further validated using a standardized IHC protocol in formalin-fixed, paraffin-embedded human tissue sections.
Immunohistochemistry antibodies are antibodies specifically designed to bind to specific antigens within tissue samples. These antibodies are usually labeled with a detectable marker, such as a fluorescent dye, an enzyme, or a radioactive label, allowing for visualization under a microscope.
Obtain immunohistochemical antibodies to visualize the distribution and localization of specific cellular components
Bind directly to the target antigen in tissues. Usually monoclonal or polyclonal antibodies.
Amerigo Scientific' immunohistochemistry antibodies have the advantages of strong specificity, accurate localization, low non-specific staining, and low background.
| Target Antigen Specificity | Antibody Type | Host Species | Tissue Type and Species | Application and Protocol Compatibility |
| Ensure that the antibodies recognize specific and unique epitopes on the target antigen. | Monoclonal vs. Polyclonal: Monoclonal antibodies are preferred due to their high specificity. |
Consider the host species in which the antibody is produced. The selection of the secondary antibody needs to be specific to the host species of the primary antibody. | Ensure the antibody has been validated for use in the specific tissue type and species you are studying. | Verify that the antibody is suitable for the specific IHC application and compatible with your experimental protocol (e.g. antigen retrieval method, fixation type). |
| Diagnostic Pathology | IHC is widely used to diagnose various diseases, including cancers, by identifying specific biomarkers and cellular changes. |
| Research | IHC can be used to study protein expression patterns, cellular localization, and tissue-specific expression, helping to understand disease mechanisms and cell biology. |
| Therapeutic Monitoring | IHC can help monitor the effectiveness of treatments by assessing changes in the expression of therapeutic targets or biomarkers. |
Amerigo Scientific offers immunohistochemical antibodies that have been tested and determined to be most suitable for use in formalin-fixed, paraffin-embedded (FFPE) tissues. Amerigo Scientific pathologists with extensive experience in IHC evaluation analyze the localization profile of each antibody to identify positive and negative cell types, signal intensity, subcellular and extracellular staining, and staining background. This information is then compared to all published expression and localization data available for that protein. This allows Amerigo Scientific to assess how each antibody behaves in IHC, including its specificity for the target protein, its detection sensitivity, and any non-specific staining features it may display. If you have a technical question, now and an experienced Ph.D. scientist can answer your questions about antibody specifications, pre-order selection, and post-order application troubleshooting.
