Fc-silenced antibodies are ideal for studies requiring precise targeting without triggering unwanted immune responses, empowering researchers in fields from immunology to oncology. At Amerigo Scientific, we offer various Fc-silenced antibody products that are designed to minimize immune effector functions.
The Fc region of an antibody plays roles in eliciting immune responses such as activating complement or binding to Fc receptors on immune cells. In some therapeutic applications, these immune responses may not be desirable or may be harmful. By silencing the Fc domain, researchers can harness the targeting abilities of the antibody without triggering these potentially detrimental immune responses.
Fc-silenced antibodies are engineered antibodies that have been modified to suppress or eliminate their Fc-mediated effector functions, like antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). These antibodies are designed to reduce unwanted immune responses that can potentially cause severe side effects in certain therapeutic or diagnostic applications. Fc-silenced antibodies are mainly used in the treatment of diseases such as immune diseases, allergies, and some forms of cancer.
Fc silencing typically involves genetic engineering techniques and strategies. For example, exchanging or swapping certain amino acids between two Fc molecules derived from different IgG subclasses is a strategy that can be employed to silence antibody effector functions. This can disrupt the binding to Fc receptors, effectively silencing the effector functions of the antibodies. Another strategy for silencing antibody effector functions is through the introduction of point mutations. By mutating one or several of these amino acids, the interaction can be disrupted, leading to a reduction or complete loss of the effector functions. The Leu234Ala/Leu235Ala (LALA) mutations are common mutations used for Fc silencing. In addition, modifying the glycosylation pattern in the Fc domain can also impact the effector functions of the antibody.
Fig.1 Effector function and binding affinity of Fc-silenced variants compared to wild-type antibodies.1
Amerigo Scientific offers a wide range of Fc-silenced antibodies by introducing key point mutations into the Fc region. Our Fc-silenced antibodies are designed to eliminate Fc-mediated effects such as ADCC and CDC while maintaining antigen-binding capabilities. With our commitment to excellence and innovation, we aim to advance your research by providing premium quality Fc-silenced antibodies and other related products at competitive prices. Our antibodies are available in different formats and suitable for various biomedical research purposes. Moreover, our experts offer custom antibody production services to meet unique requirements for your specific research projects. to find the right Fc-silenced antibodies for your needs.
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