Activating Antibody Products

Amerigo Scientific provides activating antibodies, which can be used as a class of therapeutic agents designed to bind and signal through activating receptors on effector immune cells, thereby enhancing their anticancer capabilities.

Introduction of Activating Antibodies

A desired and defining feature of an activating antibody is its ability to bind and activate the target receptor, thereby mimicking the activity of the native ligand. The propensity of an antibody to function as an activator can be influenced by several factors, including the binding epitope, affinity, valency, degree of receptor occupancy, and the interaction of the antibody crystallizable fragment (Fc) domain with Fcγ receptors (FcγRs). Unlike antagonists, which generally aim to achieve the highest possible binding affinity to competitively inhibit the ligand-binding domain of the receptor, there are no definitive rules that can predict an antibody's potential to act as an activator. Consequently, the identification and selection of activating antibodies have largely been driven by empirical functional characterization using cell-based and in vivo model systems. However, a detailed understanding of the specific stoichiometric and binding properties of the native ligand-receptor complex can aid in designing an antibody with optimal activating function against a specific receptor. Although receptor structures, ligand-binding, and signaling-engagement mechanisms are generally conserved within receptor families, they are quite unique between families.

Fig.1 Function of B7-CD28 activating antibody in T cell activation.¹

For example, the T cell-specific receptor CD28 plays a crucial role in regulating the immune system. The engagement of CD28 with its ligands, CD80/CD86, on antigen-presenting cells, delivers a crucial costimulatory signal alongside the T cell receptor for effective T cell activation. This costimulatory signal can also be mediated by the engagement of CD28 with its activating antibody.

Advantages of Our Activating Antibodies

• Enhanced Immune Activation: Activating antibodies significantly improve the activation of immune cells, leading to more effective immune responses.
• T Lymphocyte Expansion: These antibodies facilitate the proliferation of T lymphocytes within the body, which is crucial for robust anti-tumor activity.
• Tumor Cell Destruction: By enhancing immune cell activation, activating antibodies promote the efficient killing of tumor cells.
• Superior Activating Activity: They exhibit high levels of activating ability, ensuring potent receptor activation.
• Receptor Cross-Linking Capability: These antibodies possess the ability to cross-link receptors effectively, enhancing their functional efficacy.
• High Receptor Binding Affinity and Specificity: They are designed to bind with high affinity and specificity to their target receptors, ensuring precise therapeutic action.
• Batch-to-Batch Reproducibility: These antibodies maintain excellent repeatability across different production batches, ensuring consistent performance.
• High Sensitivity: They exhibit high sensitivity in detecting and binding to their target receptors.
• High Purity: Activating antibodies are produced with high purity, ensuring their safety and efficacy in clinical applications.

Antibodies Sorted by Applications

As a premier antibody supplier, Amerigo Scientific is dedicated to delivering top-tier reagents to clients worldwide. We ensure that each antibody performs as specified for the species and applications listed on our website and datasheets. Should a failure occur within the warranty period, our technical team will evaluate your experimental procedures and provide troubleshooting support. Contact us for details.