Nuclear Marker related Antibody Products

A hallmark of eukaryotic cells, the nucleus encapsulates essential genetic material. Nuclear marker antibodies serve as invaluable tools for investigating the architecture and changing dynamics of the nucleus along with its substructures. These antibodies facilitate the observation of nuclear modifications during various cellular processes. Amerigo Scientific provides a collection of nuclear marker antibodies, expertly crafted to reliably target and identify critical nuclear constituents. Each antibody undergoes rigorous validation, ensuring optimal performance for researchers delving into nuclear functions and transformations across different biological contexts.

Overview of Nuclear Markers

The nucleus, a vital membrane-bound organelle in eukaryotic cells, comprises the nuclear envelope, a double membrane that encloses genetic material and isolates it from the cytoplasm. Within this structure lies the nuclear matrix, providing structural support. Chromosomes, composed of DNA strands associated with histone proteins, house genes crucial for cellular function. Nuclear pores, spanning the impermeable nuclear membrane, facilitate selective transport of molecules. This regulation is essential for controlling gene expression, thereby orchestrating cellular activities and safeguarding genetic integrity.

Fig.1 Diagram illustrating structure of human nucleus.Distributed under Public Domain, from Wiki, without modification.

Antibodies targeting nuclear markers are invaluable tools for investigating the structure and behavior of the nucleus and its components. They enable precise monitoring of nuclear transformations during cellular activities. At Amerigo Scientific, our nuclear marker antibodies are meticulously designed to reliably detect essential nuclear targets across diverse applications, ensuring their suitability and effectiveness in research.

Applications of Nuclear Markers

• Wildlife conservation: Nuclear DNA markers hold significant promise in conservation biology for identifying protected species and assessing genetic diversity. This genetic information is crucial for detecting deteriorated populations and devising effective management plans. Accurate species classification aids in understanding biology and managing distinct populations.  Commonly used nuclear markers include RAPD, AFLP, and SSR.
• Molecular biology analysis methods: Cell type-specific nuclear markers are essential for enhancing the efficiency of FACS-based cell type isolation and improving the accuracy of single-cell nuclei bioinformatics clustering. Utilizing nuclear-specific antigens to identify cell type-specific populations in traditional methods like IHC, ICC, and FISH can streamline and enhance the efficiency and accuracy of automated image analysis pipelines.
• Cell specific nuclear marker: Using nuclear-specific antigens for identifying cell type-specific populations, as opposed to traditional whole-cell markers, can improve the efficiency, ease, and accuracy of automated image analysis pipelines. These markers can also assist in identifying and analyzing specific cell populations in novel research techniques, particularly in tissues with high cellular density and overlap. Validated single-cell populations can be utilized in downstream workflows such as RNA-seq, microarray analysis, and chromatin immunoprecipitation sequencing, enabling targeted investigations of nuclear functions and gene expression. Additionally, incorporating cell type-specific nuclear markers into bulk-tissue single-cell RNA-seq or snRNA-seq enhances the accuracy of bioinformatics clustering.

Fig.2 Human central nervous system cell types and their corresponding nuclear markers.¹

Key Nuclear Markers

At Amerigo Scientific, we offer a diverse selection of ready-to-use recombinant antibodies across multiple species, isotypes, and formats. Should you require something specific not found in our collection, our bespoke antibody engineering services are available to create precisely what you need. We encourage you to peruse our catalog of nuclear marker-related antibody products, and invite you to reach out to us with any inquiries or requests for quotes. We are eager to provide you with assistance in every possible manner.