Immunoprecipitation (IP) is a method that uses specific antibodies that bind to a target protein to isolate or concentrate a specific protein or protein complex from a sample. Amerigo Scientific offers high-quality IP/Co-IP antibodies validated against detailed experimental methods.
Immunoprecipitation antibodies are antibodies used to specifically bind and pull down a target protein or protein complex from a complex mixture. These antibodies can be monoclonal or polyclonal and are often coupled to a solid support, such as beads, to facilitate the isolation process.
Obtain an immunoprecipitation antibody to isolate the protein of interest from the sample
Amerigo Scientific offers high-quality IP/Co-IP antibodies validated against detailed experimental methods. Highly specific antibodies paired with a fully validated companion product, including IP beads and reagents, ensure reliable IP and Co-IP data to elucidate protein-protein interactions and the composition of protein complexes.
For antibodies from different host, Amerigo Scientific recommends the use of appropriate protein-conjugated beads and corresponding magnets.
The isotype control used in the IP experiment should be consistent with the IgG isoform of the primary antibody, as rabbits have only one IgG isotype, so it is relatively simple to select an isotype control.
| Specificity | Affinity | Epitope Recognition | Host Species | Compatibility with Beads |
| The antibody is selected to specifically recognize the target protein and does not cross-react with other proteins. High specificity reduces background noise and ensures that the pulled-down protein is the one of interest. | The antibody selected should have a high affinity for the target protein to ensure efficient binding. Antibodies with high affinity can capture even low-abundance proteins. | It should be ensured that the antibody recognizes an epitope that is accessible in the native (non-denatured) form of the protein, as IP typically requires the protein to be in its native conformation. | The host species that produces the antibody should be considered when selecting secondary antibody reagents and avoiding cross-reactivity with other antibodies used in downstream assays. | The antibody should be compatible with the type of beads used for IP (e.g. Protein A, Protein G, or specific antibody-conjugated beads). |
Immunoprecipitation antibodies offer a variety of advantages, and they facilitate the isolation and study of specific proteins and their interacting partners from complex biological samples. Here are the main advantages of using immunoprecipitated antibodies:
| purpose | Complexity | Analysis | Controls | |
| IP | Focuses on isolating and studying a single target protein. | Simple | Focuses on the target protein, often involving its identification, quantification, or post-translational modifications. | Typically requires controls to ensure the specificity of the antibody and the absence of non-specific binding. |
| CO-IP | Focuses on isolating and studying protein complexes, revealing interactions between the target protein and other proteins. | Complex | Extends to the proteins that co-precipitate with the target protein | Requires additional controls to validate the specificity of the observed interactions and rule out non-specific associations. |
Amerigo Scientific is known for its wide range of high-quality antibodies, with extensive validation data and experimental skills. If you have any immunoprecipitation antibody needs, please feel free to now and our experienced scientists are available around the clock to provide comprehensive datasheets, protocols, and technical support.
