Anti-idiotypic Antibody Products

Most anti-idiotypic (anti-ID) antibodies are developed to specifically target antibody drugs, providing essential tools for assessing drug efficacy, safety, and pharmacokinetics. Amerigo Scientific offers an extensive portfolio of anti-ID antibody products designed to support a wide range of biopharmaceutical applications.

Types of Anti-idiotypic Antibodies

Therapeutic monoclonal antibodies and biosimilars have a significant impact on the treatment of many diseases. However, evaluating the safety and efficacy of these drugs is crucial in the drug development process. An anti-ID antibody is a specific antibody that recognizes and binds to the variable region of another antibody, known as the idiotype. Anti-ID is a valuable tool in various research and clinical applications, enabling researchers to study antibody-drug interactions, monitor drug levels, and evaluate immune responses to therapeutic antibodies. Anti-IDs can be used in pharmacokinetic assays to measure the concentration of therapeutic antibodies in the blood or tissues. They also can serve as positive controls for detecting and quantifying anti-drug antibody (ADA) responses to biologic therapeutics or monoclonal antibodies.

Anti-ID antibodies can be classified into three types according to different binding modes and properties. Based on these characteristics, different anti-ID antibodies can be used in PK assays to determine free, total, or bound concentrations of antibody drugs.

• Type 1: Inhibitory anti-ID antibodies directly compete with the antigen and effectively block antigen binding. They can detect free antibody drugs.
• Type 2: Non-inhibitory anti-ID antibodies can detect bound, partially bound, and free antibody drugs.
• Type 3: Complex-specific anti-ID antibodies only bind to the antibody drug that is bound to its antigen. Therefore, these anti-IDs detect bound antibody drugs.

Applications

Anti-idiotype antibodies have various applications in research, diagnostics, and therapeutic development. Common applications of anti-idiotype antibodies include:

• PK Assay: Anti-idiotype antibodies can be used as reagents in pharmacokinetic (PK) assays to assess the presence and concentrations of therapeutic antibodies in biological samples. By binding to the idiotype region of the target antibody, these anti-idiotype antibodies help detect and quantify the levels of the therapeutic antibody.
• ADA Assay: Anti-drug antibody (ADA) assays are used to detect and measure the immune response to therapeutic antibodies. Anti-idiotype antibodies can serve as positive controls in ADA assays, mimicking the binding of immunogenic epitopes on the therapeutic antibody and aiding in assay development and validation.

Product Range

Amerigo Scientific offers various off-the-shelf anti-ID antibodies that allow researchers to select the most suitable reagent for their specific experimental needs, facilitating a wide range of scientific investigations in the fields of immunology, oncology, and infectious diseases. With their specificity and versatility, our anti-ID antibodies serve as valuable tools for assessing pharmacokinetics, drug levels, and immune responses. Our team of experienced scientists also provides customization services to tailor anti-ID antibodies to meet specific research requirements, ensuring optimal performance and accuracy.