The enzyme linked immunosorbent assay (ELISA) is a highly sensitive assay based on an immune response. It works by combining the specific reactions of antigens and antibodies with the efficient catalytic action of enzymes on substrates. Amerigo Scientific offers a wide range of high-quality antibodies validated by ELISA and other immunoassays, as well as detailed protocols, for a wide range of targets and applications.
ELISA and immunoassay antibodies are specialized antibodies used to detect and quantify specific antigens in various sample types. These antibodies are integral components of immunoassays, where they provide the specificity and sensitivity needed for accurate measurement of target molecules.
Uses a primary antibody directly conjugated to an enzyme that reacts with a substrate to produce a detectable signal; Simple and fast.
Involves an unlabeled primary antibody specific to the target antigen, followed by an enzyme-conjugated secondary antibody that binds to the primary antibody; Provides higher sensitivity and flexibility.
Utilizes two antibodies: a capture antibody bound to the plate that captures the target antigen, and a detection antibody that binds to the captured antigen; Highly specific and sensitive, ideal for complex samples.
Involves competition between the sample antigen and a labeled antigen for binding to a limited amount of antibody; Suitable for small molecules and antigens with a single binding site.
Obtain ELISA and immunoassay antibodies for quantitative detection of targets
| High Sensitivity and Specificity | Quantitative Results | Versatility | High Throughput | Compatibility with Various Sample Types |
| ELISA and immunoassays can detect very low concentrations of antigens. Specific antibodies ensure accurate identification of the target antigen, reducing the likelihood of false positives and false negatives. | ELISA and immunoassays provide quantitative results that allow for precise measurement of antigen concentrations in a sample. | ELISAs and immunoassays can be used to detect a wide range of targets, including peptides, proteins, viruses, hormones, and small molecules. | Some immunoassays enable multiplexing with the ability to detect multiple targets simultaneously in a single assay. | ELISA and immunoassays are suitable for the detection of a wide range of sample types, including blood, plasma, serum, saliva, urine, cell lysates, and tissue homogenates. |
| Specificity | Sensitivity | Validation | Antibody Pairing | Host Species and Isotype |
| Choose antibodies with high specificity to the target antigen to minimize cross-reactivity. | Select antibodies with high affinity to ensure sensitive detection of low-abundance antigens. | Use antibodies validated for ELISA and other immunoassays to ensure reliable performance. | For sandwich ELISAs, it is important to ensure that the capture and detection antibodies bind to the different epitopes on the antigen. | Consider the host species and isotype of the antibodies for compatibility with the detection system and other assay components. |
Amerigo Scientific is known for its high-quality products and support, making it a reliable choice for your research and diagnostic needs. We offer a wide range of high-quality antibodies validated by ELISA and other immunoassays. We also provide detailed datasheets and quality control data to help customers select the best antibody for their experiments. If you have any questions about enzyme-linked immunosorbent assays and related product selection, please feel free to !
