Anti-HTT Monoclonal Antibody (3B5H10)

Subcat	Description	Isotype	Antibody Modification	Conjugate	Price	Availability
ASPC007	Anti-HTT Antibody (3B5H10)	Mouse IgG1, Lambda		None	$460	4-6 weeks	Add
ASPC007-2	Anti-HTT Antibody (3B5H10), scFv fragment	Mouse scFv	His tag	None	$460	In stock	Add
ASPC007-P	Anti-HTT Antibody (3B5H10), PE	Mouse IgG1, Lambda		PE	$1,180	2-3 weeks	Add
ASPC007-A	Anti-HTT Antibody (3B5H10), APC	Mouse IgG1, Lambda		APC	$980	2-3 weeks	Add
ASPC007-H	Anti-HTT Antibody (3B5H10), HRP	Mouse IgG1, Lambda		HRP	$600	2-3 weeks	Add
ASPC007-B	Anti-HTT Antibody (3B5H10), Biotin	Mouse IgG1, Lambda		Biotin	$680	2-3 weeks	Add

Anti-HTT Monoclonal Antibody (3B5H10)
- Product Description:
  
  3B5H10 specifically binds to polyglutamine (polyQ) tracts. These expanded polyQ tracts are associated with the pathogenesis of Huntington's disease and other polyglutamine disorders. This antibody does not recognize normal, non-pathogenic forms of huntingtin, making it particularly useful for detecting disease-relevant protein forms.
  
  Applications:
  
  Immunoprecipitation, Immunofluorescence, Electron Microscopy, Immunohistochemistry, Inhibition, Western Blot
  
  Source:
  
  Mouse
  
  Target:
  
  HTT
  
  Target Species:
  
  Human
  
  Specificity:
  
  This antibody recognizes extended polyglutamine repeats (polyQ) in mutant huntingtin.
  
  Immunogen:
  
  A fragment of human Huntingtin, consisting of the N-terminal 1-171 amino acids, with a polyglutamine stretch of 68 residues.
  
  Clonality:
  
  Monoclonal
  
  Clone ID:
  
  3B5H10
  
  Isotype:
  
  Mouse IgG1, Lambda
  
  Purification:
  
  The antibody was purified by Protein A.
  
  Purity:
  
  >95% as determined by SDS-PAGE
  
  Aggregation:
  
  <5% as determined by SEC
  
  Concentration:
  
  1 mg/mL
  
  Form:
  
  Liquid
  
  Endotoxin:
  
  Please contact us for more information.
  
  Formulation:
  
  Contact us for customized product form or formulation.
  
  Preservative:
  
  BSA and Azide free.
  
  Stabilizer:
  
  None
  
  Shipping:
  
  Shipped at 4°C.
  
  Storage:
  
  This antibody can be store at 2°C-8°C for one month. Upon delivery aliquot. For longer storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Anti-HTT Monoclonal Antibody (3B5H10) Applications
- Note:
  
  Optimal dilutions/concentrations should be determined by the end user.

Gene Symbol

HTT

Protein Name

Huntingtin

Introduction

Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range of trinucleotide repeats (9-35) has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression.

Alternative Names

Huntingtin; Huntington Disease Protein; IT15; HD; Huntingtin (Huntington Disease); HD Protein; LOMARS

Gene ID

3064

UniProt

P42858

Related Disease

Huntington disease (HD)

Subcellular Location

Nucleoplasm,Cytosol

Tissue Specificity

Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.

Cell Type

B cells, T cells, Thymocytes, Tregs

Function

May play a role in microtubule-mediated transport or vesicle function.

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