Medium-Salt Active Nuclease High Quality ( M-SAN HQ )

- Overview
  - M-SAN HQ is the latest addition to our high salt tolerance nucleases portfolio. The biochemical properties of the SAN family of nucleases make them ideal for multiple bioprocessing and biomanufacturing workflows.
    M-SAN HQ has been developed to offer the optimal solution for removal of nucleic acids at the near-physiological conditions used in many bioprocessing and biomanufacturing workﬂows. This novel, nonspeciﬁc endonuclease is active over a broad pH range and displays optimum activity at salt concentrations around 175 mM.
    
    The main advantages of M-SAN High Quality
    • High purity (≥ 98%)
    • No protease detected
    • Animal origin-free production
    • Endotoxin-tested
    • High activity at physiological salt and pH
    • Supplied with extended product documentation
    
    Please contact us at for specific academic pricing.
    
    More Details
    
    Nucleases
- Properties
  - Source
    
    Pichia pastoris
    
    Type
    
    Recombinant
    
    Note
    
    • Tolerance to typical buffer additives:
    o DTT and other reducing agents may inactivate M-SAN HQ
    o Urea: Not recommended
    o EDTA: Not recommended
    
    Concentration
    
    25 kU: 25 - 30 U/µl
    500 kU: ≥ 250 U/µl
    5 MU: ≥ 250 U/µl
    
    Molecular Weight
    
    Protein size is 24.5 kDa.
    
    Activity
    
    • Temp: 25–40°C, optimal 37°C
    • NaCl: Working range (>20%): 50-400mM, optimal range (>80%): 125mM–250mM
    • Mg2+ (>1mM) is required for activity, optimal range Mg2+ is >4 mM
    • pH: Working range (>20%): 6.5-10.0, optimal range (>80%): 7.2–9.5
    
    * For research use only
- Applications
  - Application
    
    Viral Bioprocessing
    
    Application Description
    
    M-SAN High Quality for Bioprocessing is a nonspecific endonuclease that is highly active at physiological conditions. M-SAN High Quality can be used directly in expression media, which can improve efficiency and yield in various workflows.